Abstract
Immediately after dental implant insertion, blood will be in direct contact and interact with the implant surface and activates inflammatory responses and complement cascades within seconds. The aim of the present study was to determine the ability of fluoride-modified titanium surfaces to activate complement cascades using the human buffy coat as model. The buffy coats were exposed to hydrofluoric acid-modified surfaces for a short time and its responses were compared to controls. Identification and quantification of complement cascade biomarkers were conducted using ELISA kits and multianalyte profiling using Luminex. A lower level of C3 at 30 min and increased levels of C4, MIP-4, CRP, and pigment epithelium-derived factor at 360 min were found on modified surfaces as compared to controls. We found no significant differences in the levels of C3a, C5a, C Factor H, α2M, ApoA1, ApoC3, ApoE, Prealbumin, α1AT, and SAP in modified surfaces in the buffy coats. We conclude that titanium surfaces treated with hydrofluoric acid modify the levels of specific biomarkers related to the complement cascade and angiogenesis and, thus, tissue growth, remodeling and repair, as this may play a role in the enhanced clinical performance of fluoride-modified Ti dental implants.
Highlights
More than 5 million dental implants are inserted yearly in the U.S alone, according to Grand ViewResearch
We have previously studied the effect of fluoride treatment on human primary osteoblasts and human gingival fibroblasts [14,15], without different cellular responses to fluoride and non-fluoride treated surface
A lower level of C3 was found after exposure to TiO2 surface (TiF) surfaces compared to non-modified surfaces at earliest time point, 30 min (p < 0.05)
Summary
More than 5 million dental implants are inserted yearly in the U.S alone, according to Grand ViewResearch. Fluoride treated surface dental implant (Osseospeed®, Dentsply, York, PA, USA). Fluoride-treated dental implants have shown clinically successful results with increased bone-to-implant contact and functional bone attachment [4,5,6,7,8]. It has been well reported that fluoride improve the peri-implant tissue responses [9,10,11,12,13]. Despite these satisfactory clinical results, the identification of molecular mechanisms behind the success is still unidentified. We have previously studied the effect of fluoride treatment on human primary osteoblasts and human gingival fibroblasts [14,15], without different cellular responses to fluoride and non-fluoride treated surface
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
