Fluoresence Studies of Nucleotides and Polynucleotides: III. DIPHOSPHOPYRIDINE NUCLEOTIDE ANALOGUES WHICH CONTAIN FLUORESCENT PURINES

Abstract

Diphosphopyridine nucleotide analogues have been prepared in which the adenosine 5′-monophosphate of the coenzyme is replaced by the 5′-monophosphate of one of the following fluorescent nucleosides: formycin, 2-aminopurine riboside, and 7-deazapurine riboside (7-deazanebularin). The analogue compounds, unlike the parent coenzyme, are highly fluorescent in both oxidized and reduced form. Their spectral and fluorescence properties have been examined as a function of temperature, pH, and solvent conditions. All three analogues show significant coenzyme activity and can replace DPN with all of the five dehydrogenases tested; the efficiency of the analogues in these tests is in the range of 20 to 150%, compared with DPN+ itself.