Abstract
ABSTRACT The aim of this paper was to characterize and compare spitting cobras of Africa. The autofluorescence of Naja ashei and Naja nigricollis venoms was investigated by atomic force microscopy and fluorescence analysis. The synchronous fluorescence analysis showed higher endogenous fluorescence of Naja ashei venom in comparison with Naja nigricollis venom. The atomic force microscopy revealed different surfaces of compared venoms as a result of different amounts of proteins. Our results confirmed different structures of Naja nigricollis and Naja ashei venom. The practical advantages of selected techniques are high sensitivity, minimal quantity of venom, and capability to measure the spitting cobra by autofluorescence.
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