Abstract
A method employing fluorimetry and spectrophotometry on eluates from a single paper chromatogram was devised for the rapid analysis of chlorogenic plus neochlorogenic acids, rutin, 6-hydroxykynurenic acid, scopolin and scopoletin in flue-cured tobacco and was applied to fifty-six carefully graded samples from the 1964–65 North Queensland crop. Quality, as determined by the minimum price set for each grade, showed no overall correlation with the content of any of these compounds. Within a given leaf colour class there was a significant negative correlation of quality with scopoletin content. Within a given leaf position class there were no correlations. CGA, rutin and scopoletin content varied with leaf position, i.e. showed distribution gradients within the plant. Only CGA content varied with colour. The levels of CGA, rutin and 6-HKA were positively correlated with each other but not with those of scopolin or scopoletin.
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