Abstract
A new fluorescent peptidyl chemosensor for Cu 2+ ions with fluorescence resonance energy transfer (FRET) capabilities has been synthesized via Fmoc solid-phase peptide synthesis. The metal chelating unit, which is flanked by the fluorophores tryptophan (donor) and dansyl chloride (acceptor), consists of the amino acids glycine and aspartic acid (Gly-Gly-Asp-Gly-Gly-Asp-Gly-Gly-Asp-Gly-Gly-Asp-Gly-Gly). Coordination of the Cu 2+ ions to the metal chelating unit results in fluorescent quenching of both the donor and acceptor fluorophores. Although it was determined that Cu 2+ binding causes no change in FRET efficiency, emission and Cu 2+-induced quenching of the acceptor dye can be used to monitor the concentration of the copper ions, with a detection limit of 32 μg L −1. The sensor also demonstrated sensitivity, reversibility and selectivity towards Cu 2+ in a transition metal matrix at pH 7.0.
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