Abstract
This study was prompted by the well-known problem concerning unsuccessful detection of male DNA profiles in unfavorable mixtures of male and female cells. Different mixtures of male and female cell samples were stained, using a fluorescence-in-situ-hybridisation kit (Vysis), which includes different color probes for the X- and the Y-chromosome. The male cells were isolated via the SL μCut LMD system from Molecular Machines and Industries AG (MMI). STR profiling of hybridized cells enabled a full profile to be obtained from samples containing at least 30 cells. Samples with 20 cells yield a partial profile with one or two locus dropouts.
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