Abstract
A fluorescent fullerene nanoparticle (NP)-based lateral flow immunochromatographic assay (LFIA) was developed for the rapid and quantitative detection of C-reactive protein (CRP) in serum. The polyclonal CRP-antibody-conjugated fullerene NPs were simply prepared by 1-ethyl-3-(3-dimethyllaminopropyl)-carbodiimide hydrochloride coupling after carboxylation of fluorescent fullerene NPs. By applying the CRP-antibody-conjugated fullerene NPs to a lateral flow test strip, quantitative analysis of CRP in serum was possible at a concentration range of 0.1–10 ng/ml within 15 min. We anticipate that this novel fluorescent fullerene NP-based LFIA can be useful for the rapid and accurate sensing of biological and chemical species, contributing to the disease diagnosis and prognosis, environmental monitoring, and food safety.
Highlights
The lateral flow immunochromatographic assay (LFIA) is a common technique for the detection of such diverse analytes as hormones, disease-related biomarkers, and toxins in the clinical, environmental, and food industry fields, because of its simplicity and rapidity [1,2,3,4,5,6,7,8]
To overcome the drawbacks of colloidal gold (CG)-based LFIA, various materials have been developed as reporters, including fluorescent microspheres (FMs), quantum dots (QDs), up-conversion nanoparticles (UCNPs), carbon nanoparticles (CNPs), and platinum nanoparticles (PtNPs) [9,10,11,12,13]
C-reactive protein (CRP)-C60-tetraethylene glycol (TEG) preparation process is uncomplicated and easy to perform under ambient conditions, whereas other reporting materials, e.g., semiconducting QDs and
Summary
The lateral flow immunochromatographic assay (LFIA) is a common technique for the detection of such diverse analytes as hormones, disease-related biomarkers, and toxins in the clinical, environmental, and food industry fields, because of its simplicity and rapidity [1,2,3,4,5,6,7,8]. To overcome the drawbacks of CG-based LFIA, various materials have been developed as reporters, including fluorescent microspheres (FMs), quantum dots (QDs), up-conversion nanoparticles (UCNPs), carbon nanoparticles (CNPs), and platinum nanoparticles (PtNPs) [9,10,11,12,13]. Park et al Nano Convergence (2019) 6:35 protein that is a non-specific but sensitive inflammation marker, especially in the case of bacterial infection It is known as a potential indicator of cardiovascular disease, e.g., coronary heart disease, ischemic stroke, and acute myocardial infarction [16,17,18]. Since the developed C60-TEG-based LFIA achieves sufficiently high sensitivity and quantitative analysis of a target molecule, the C60-TEG-based LFIA can be used as an advanced fluorescent LFIA for disease diagnosis and prognosis, environmental monitoring, and food safety
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