Abstract
Calcofluor white M2R is useful as a nonspecific fluorescent stain for the identification of fungi. In tissues, calcofluor white M2R is used commercially as an additive to detergents that make clothes appear “whiter.” Many other compounds are also used in this fashion. These other whitening compounds were examined for their ability to stain fungi in frozen and paraffin-embedded tissue. All compounds were effective to some degree, but Tinopal CBSX was especially effective. The fluorescence of fungi stained with Tinopal was subjectively and objectively more intense than that using calcofluor white M2R.
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