Fluorescense Study of the Interaction Between Adenosine Disodium Triphosphate (ATP) and Ciprofloxacin‐ Tb3+ and Its Analytical Application

Abstract

A new spectrofluorimetric method was developed for determination of adenosine disodium triphosphate (ATP). We studied the interactions between ciprofloxacin (CIP)‐ Tb3+ complex and adenosine disodium triphosphate (ATP) by using UV–Visible absorption and fluorescence spectra. Using CIP‐ Tb3+ as a fluorescence probe, under the optimum conditions, ATP can remarkably enhance the fluorescence intensity of the CIP‐Tb3+ complex at λ = 545 nm and the enhanced fluorescence intensity of Tb3+ ion is in proportion to the concentration of ATP. Optimum conditions for the determination of ATP were also investigated. The linear ranges for ATP are 1.00 × 10−7∼1.00 × 10−5 mol/L with detection limits of 4.60 × 10−8 mol/L. This method is simple, practical, and relatively free of interference from coexisting substances and can be successfully applied to determination of ATP in samples. The mechanism of fluorescence enhancement between CIP‐ Tb3+ complex and ATP was also studied. The authors are grateful to the National Natural Science Foundation of China for its financial support. (Grant No. 20271043).