Abstract
A homogeneous fluorophore-linked binding assay for biotin and biotin-containing compounds has been used in a merging-zone flow-injection system. Analyses were first conducted by taking advantage of the enhancement in fluorescence intensity of a conjugate of avidin-fluorescein in the presence of biotin or biotin derivatives. Detection limits for biotin as low as 2 × 10 −9 M could be achieved. A greater enhancement of fluorescence was found for a streptavidin-fluorescein conjugate. Calibration curves obtained by using the streptavidin conjugate are shown for the determination of various biotinylated compounds in the flow-injection system and in a batch mode.
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