Fluorescence turn-on strategy based on silver nanoclusters-Cu2+ system for trace detection of quinolones

Abstract

Herein, utilizing fluorescent Ag nanoclusters (Ag NCs) capped by polyethyleneimine (PEI) with different molecular weights, we constructed a novel Ag NCs-Cu2+ sensing system based on fluorescence turn-on strategy for trace detection of quinolones, including nalidixic acid, cinoxacin, ciprofloxacin/ofloxacin, and moxifloxacin; these were the representatives of four generations of quinolone antibacterial drugs. The fluorescence of Ag NCs could be efficiently quenched by Cu2+, while with the addition of quinolones, the fluorescence of this system recovered rapidly. This protocol exhibited excellent sensitivities to these five quinolones. For instance, the linear ranges were from 1 to 100nM for nalidixic acid with limit of detection (LOD) 0.47nM, from 1 to 80nM for cinoxacin with LOD 0.43nM, from 0.1 to 120nM for ciprofloxacin with LOD 38pM, from 0.1 to 80nM for ofloxacin with LOD 49pM, and from 0.1 to 120nM for moxifloxacin with LOD 48pM, respectively, revealing that this approach showed low detection limits and wide linear ranges. Benefiting from the high sensitivity, the satisfactory recoveries were obtained for trace detection of quinolones in real samples, such as tablets, human urine and serum.