Fluorescence turn-on sensing of L-cysteine based on FRET between Au-Ag nanoclusters and Au nanorods.

Abstract

The applications of metallic nanoclusters and nanoparticles in biological sensing have attracted special attention owing to their optical interaction based on fluorescence and surface plasmon resonance (SPR). In this work, we designed a fluorescent nanoprobe for the determination of L-cysteine (L-Cys) based on fluorescence resonance energy transfer (FRET) from gold‑silver bimetallic nanoclusters (Au-Ag NCs) to gold nanorods (AuNRs). Firstly, the negatively charged Au-Ag NCs protected by bovine serum albumin (BSA) are directly adsorbed on the surface of the positively charged AuNRs through electrostatic interaction, and the FRET effect leads to distinct fluorescence quenching of Au-Ag NCs at 615nm. The SPR wavelength of AuNRs is dependent on the aspect ratio, so the SPR of AuNRs could be tuned to have a better spectral overlap with fluorescence of Au-Ag NCs, which enhances the fluorescence quenching effect. Because the SH group of L-Cys has an affinity with gold, the addition of L-Cys can result in the release of Au-Ag NCs from the surface of AuNRs via forming AuS bonds. Thus, the introduction of L-Cys could effectively restore the fluorescence emission of the AuNRs/Au-Ag NCs system because of the restraint of FRET effect. Under the optimized conditions, the fluorescence recovery of AuNRs/Au-Ag NCs probe exhibits a linear response to L-Cys concentration ranging from 5 to 100μM, and the corresponding theoretical detection limit (LOD) is 1.73μM. Meanwhile, this method displays excellent sensitivity and selectivity for L-Cys over other amino acids, and it has been successfully applied to detect L-Cys in real samples.