Abstract
Keywords: fluorescence spectroscopy, fluorescence correlation spectroscopy, distribution analysis of depth-dependent quenching, annexin, diphtheria toxin, Bcl-xL
Highlights
The transition of a soluble protein into a lipid membrane is of fundamental significance in a number of physiological and disease processes
Notable examples include the cellular entry of bacterial toxins, colicins, and viruses, and apoptotic regulation by the Bcl-2 protein fa mily
Additional details on the applications of fluorescence spectroscopy in the membrane protein studies can be found in the following recent reviews, describing thermodynamic measurements of membrane bin ding [11] and determination of the depth of membrane penetration by proteins and peptides [12]
Summary
The transition of a soluble protein into a lipid membrane is of fundamental significance in a number of physiological and disease processes. We will show how fluorescence-based approaches can be applied to monitor different aspects of protein membrane insertion, including kinetic pathways of membrane insertion and free energy landscapes, specific conformational changes, structure and topology of membrane-inserted states, as well as elucidating the role of lipids on the membrane protein insertion pathways. Additional details on the applications of fluorescence spectroscopy in the membrane protein studies can be found in the following recent reviews, describing thermodynamic measurements of membrane bin ding [11] and determination of the depth of membrane penetration by proteins and peptides [12]
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