Abstract

AbstractAutofluorescence of the mandible and femur bones taken from newborn rats (7-, 14- and 28-day old) has been studied. Endogenous fluorophores were excited with 291, 340 and 360 nm wavelengths. Modifications in content and microenvironment of both noncolagenous and collagenous constituents of bone tissue as well as metabolic coenzymes during the bone formation are reflected in fluorescence emission spectra. The biggest changes in fluorescence intensity were observed for tryptophan present in noncollagenous proteins. The most significant changes for collagen and metabolic coenzymes were blue shifts of emission peak maximum. The dependence of the emission spectra on the rat age was found.Keywordsendogenous fluorophoresautofluorescencerat mandiblerat femurbone development

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