Abstract
Histamine (His) is used as an indicator of seafood quality, but it can be toxic at high intakes. A fluorescence (FL)-surface-enhanced Raman scattering (SERS) dual-mode assay system has been developed for His detection. The His detection method was established based on the specific binding capacity of gold nanoparticles (AuNPs) for the FL derivative of His and o-phthalaldehyde (OPA). In this strategy, His reacted with the OPA to form a Schiff base product (O-His) along with a change in FL and SERS activities. The usual nature of AuNPs could display a significant role both enhancement of SERS and quenching of FL signals. The current investigation displayed a good selectivity toward His over all other biogenic amines. Under the optimized analytical conditions, the SERS and FL intensity of the system were linearly proportional to the His concentration in the range of 0.05–4.5 mg/L and 1–20 mg/L with a detection limit of 0.04 mg/L and 0.32 mg/L, respectively. Moreover, the proposed method was successfully applied for His determination in seafood with promising results.
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