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Abstract
A four-colour set of fluorescence resonance energy transfer dideoxy nucleotide terminators ( 10– 13), have been synthesised using a rigid and linear tri-functional molecule ( 2), synthesised via Heck coupling reaction of t-Boc- l-4-iodophenylalanine ( 1) with N-TFA-propargylamine. Evaluation of the terminators in DNA sequencing reactions, in combination with Thermo Sequenase™ II DNA polymerase, demonstrated them to be excellent reagents for high-throughput DNA sequencing.
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