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Abstract
Bi2Se3, ZnS, and ZnSe nanoparticles were synthesized and their surface modified using tetraethyl orthosilicate and (3‐aminopropyl) tetraethyl orthosilicate. They were conjugated to an anti‐EGFR antibody. SEM images indicate that the sizes of the modified nanoparticles (NPs) Bi2Se3/SiO2, ZnS/SiO2, and ZnSe/SiO2 are about 20 nm. Förster‐Fluorescence resonance energy transfer (FRET) between the anti‐EGFR antibody and ZnS/SiO2, ZnSe/SiO2, and Bi2Se3/SiO2 nanomaterials was investigated in‐depth showing e.g. that with conjugation of the anti‐EGFR to nanomaterials, fluorescence intensity increases. This increase reaches up to 6.6 times for ZnS/SiO2‐anti‐EGFR conjugate NPs making their sensitivity attractive for biosensor applications. Fluorescence lifetimes were also increased for all conjugated NPs with markedly higher lifetime for Bi2Se3/SiO2 compared with ZnS/SiO2 and ZnSe/SiO2.
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