Fluorescence reaction ribonucleosides and ribonucleotides with 1,2-bis(4-methoxyphenyl)ethylenediamine.

Abstract

Ribonucleosides and ribonucleotides produce fluorescence (excitation maxima, ca. 340 nm; emission maxima, ca. 470 nm) when heated in an acidic solution with meso-1,2-diarylthylenediamines which have phenyl groups substituted with electron-donating groups at the 4-positions, after periodate oxidation. Of the1,2-diarylthelynediamines tested, meso-1,2-bis(4-methoxyphenyl)ethylenediamine was the most sensitive and permitted the fluorometric determination of ribonucleosides and ribonucleotides at concentrations as low as 60-500 pmol/ml