Abstract
Using as an example the concentration dependence of the fluorescence quenching constants for quenching by Cd2+ ions for different weight fractions of humic acid, we have demonstrated the application of the fluorescence quenching method for studying the effect of association on the structure of humic acids. We have established that the quenching constants for the light fraction (5–30 kD) decrease as the humic acid concentration increases and the quenching constants increase for the heavy fraction (60–100 kD). The observed dependences are interpreted in terms of the idea of oppositely directed effects of association on the structure of the environment of fluorophores located within the core and peripheral regions of the humic acid macromolecule.
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