Abstract
We have prepared a partially-conjugated water-soluble polymer by simply heating a solution of PPV precursor polymer. The partially conjugated polymer2 obtained by this method was strongly fluorescent and the fluorescence was quenched by proteins such as bovine serum albumin, α-chymotrypsin, and hemoglobin. Hemoglobin was found to be superior to other proteins investigated in terms of quenching ability. The fluorescence quenching of the conjugated polymer2 by hemoglobin is presumably due to the electron transfer from the polymer to heme moieties in the protein. Patterned fluorescence images were readily obtained by spotting hemoglobin on a PPV film by employing a modern array technology.
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