Abstract
Abstract The fluorescence quenching of 1-pyrenemethanol (PyM) by serotonin (Ser) in polystyrene latex dispersions has been studied by steady-state and time-resolved fluorescence spectroscopy. From steady state fluorescence measurements, it is found that the quenching is remarkably enhanced on going from an aqueous homogeneous solution to a latex dispersion. This is attributed to that both PyM and Ser are effectively adsorbed onto the latex particles, as evidenced by measurements of the adsorption isotherms. Fluorescence decay curves of PyM in polystyrene latex dispersions have been successfully analyzed in terms of double exponential functions. The analysis reveals that the quenching of PyM fluorescence by Ser on polystyrene latex surfaces is mainly operated by a static mechanism, although the contribution of a dynamic mechanism is not negligible.
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