Abstract
Humic acids as a water soluble fraction of humus play important role in the plant growth. Even though structure of humic acids is not clear, their spectroscopic properties can reveal us useful information. To investigate influence of humic acids reorganization, HPSEC fractionation at mild fractionation conditions has been carried out. Reconstruction of secondary chromatograms of separated fractions showed molecular size increase and increased response on fluorescence detector, although neither molecular size change nor significant absorbance increase was observed on UV-VIS detector. However, bigger aggregates, that didn't fluoresce in the unfractionated sample started to fluoresce. The reason for this behavior is not some change of spectral properties of a specific fraction, but a general fluorescence increase indicating humic acid reorganization. Therefore spectroscopic changes can be used as a tool for monitoring their reorganization, which might play an important role in nutrients soil plant mobility and should be studied in more details from that perspective.
Highlights
In soil science humus refers to any organic matter that has reached a point of stability, where it will break down no further
Reconstruction of secondary chromatograms of separated fractions showed molecular size increase and increased response on fluorescence detector, neither molecular size change nor significant absorbance increase was observed on UV-VIS detector
Analyzes of Humic acids (HA) samples were performed by aqueous high pressure size exclusion chromatography (HPSEC) using a high pressure liquid chromatograph Agilent HP 1100 Series with
Summary
In soil science humus refers to any organic matter that has reached a point of stability, where it will break down no further. Stable (or passive) humus consists of humic acids and humins (DiGiovanni et al, 1998). Humic acids (HA) as a water soluble fraction of humus play important role in the plant growth (Lee and Bartlett, 1976; Nardi et al, 2002). The beneficial effects of HA on plant growth may be related to their indirect (increase of fertilizer efficiency or reducing soil compaction), or direct (improvement of the overall plant biomass) effects. They play important role in ion absorption (increase the availability of micronutrients from sparingly soluble hydroxides (Stevenson, 1994)). Apart from that, HA are absorbed by plants, influencing intermediary metabolism, or plant growth and development directly, by acting as hormone-like
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