Abstract
In 32 healthy blood donors, 20 patients with histologically verified cancer and 18 patients with non-neoplastic diseases, the fluorescence polarization changes of fluorescein samples incorporated in mononuclear leucotyes were measured after incubation with PHA. The leucocytes of healthy persons, and 16/18 persons with non-neoplastic diseases, responded with a decrease in the degree of fluorescence polarization by about 20% from that in non-PHA-stimulated cells. In 19/20 patients with a variety of malignant tumours, the leucotyes did not respond to PHA stimulation with such a decrease. The exceptions among the patients with neoplastic and non-neoplastic diseases are considered, and may not be "false-negative" or "false-positive" respectively, but indicative of a particular situation in that disease. The biophysical mechanisms underlying the observed changes remain to be investigated.
Highlights
Summary.-In 32 healthy blood donors, 20 patients with histologically verified cancer and 18 patients with non-neoplastic diseases, the fluorescence polarization changes of fluorescein samples incorporated in mononuclear leucocytes were measured after incubation with PHA
Our studies show that there are fluorescence-polarization changes in mononuclear blood cells after their incubation with PHA which are absent when cells of the same density characteristics from patients with neoplasia are investigated
Cercek et al (1974,) and Cercek and Cercek (1974, 1975, 1976) reported on the possibility of measuring the response of lymphocytes to the actions of mitogens such as PHA or to cancer basic protein (CaBP) by means of fluorescence-polarization changes. They reported that lymphocytes from normal huiman beings and from patients with non-neoplastic diseases responded to PHA stimulation wTith an immediate decrease in fluorescence polarization, which decrease was absent from lymphocytes from patients with neoplastic diseases
Summary
From the *Department of Clinical Physiology and the tDepartrnent of Experimental Physics III, University of Ulnm, Federal Republic of Germany. Summary.-In 32 healthy blood donors, 20 patients with histologically verified cancer and 18 patients with non-neoplastic diseases, the fluorescence polarization changes of fluorescein samples incorporated in mononuclear leucocytes were measured after incubation with PHA. The biophysical mechanisms underlying the observed changes remain to be investigated It has been the purpose of our studies to investigate the influence of phytohaemagglutinin (PHA) on the fluorescence polarization of fluorescein samples incorporated into mononuclear blood cells of normal human beings, of patients with histologically confirmed neoplasia, and of patients with non-neoplastic diseases. Our studies show that there are fluorescence-polarization changes in mononuclear blood cells after their incubation with PHA which are absent when cells of the same density characteristics from patients with neoplasia are investigated. The mechanisms that lead to the observed changes are far from being clear, and require further investigation
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