Abstract
Detection of ciprofloxacin residues in milk by sensitive and rapid methods is of great interest due to its use in the treatment of dairy livestock health. Current analytical approaches to antibiotics detection, are laboratory-based methods and they are time-consuming and require trained personnel. To cope this problem, we propose an assay, based on fluorescence polarization principle, able to detect the presence of ciprofloxacin in diluted milk sample without any pre-treatment. The proposed method is based on the use of ciprofloxacin-protein conjugate labeled with near infrared fluorescence dye, which upon binding to specific antibody causes an increase of the fluorescence polarization emission signal. The developed assay allows for the detection of ciprofloxacin at a concentration of 1ppb, which represents an amount lower than the maximum residual limit (MRL) of ciprofloxacin in milk, as set by the European Union regulation (100 ppb).
Highlights
Detection of ciprofloxacin residues in milk by sensitive and rapid methods is of great interest due to its use in the treatment of dairy livestock health
Among anti-bacterial agents used for the management of infections in dairy livestock, ciprofloxacin, belonging to the fluoroquinolone class, is the common antibiotic used in case of pulmonary, urinary and digestive infections[6]
We describe a fluorescence polarization assay for the detection of the ciprofloxacin
Summary
Detection of ciprofloxacin residues in milk by sensitive and rapid methods is of great interest due to its use in the treatment of dairy livestock health. Current analytical approaches to antibiotics detection, are laboratory-based methods and they are time-consuming and require trained personnel. To cope this problem, we propose an assay, based on fluorescence polarization principle, able to detect the presence of ciprofloxacin in diluted milk sample without any pre-treatment. The antibiotics treatment plays a crucial role in dairy livestock health[1] It is being used for therapeutic purposes as well as for improving breeding efficiency. Laboratory-based methods, such as liquid chromatography-mass spectrometry (LC-MS), high performance liquid chromatography (HPLC)[11,12,13] and microbiological assays[14] are the common used approaches for ciprofloxacin detection. It is worth to note that this method can be transferred into a hand-held device capable to acquire fluorescence polarization changes when ciprofloxacin derivative and anti-ciprofloxacin antibodies interacts
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