Abstract
The mould Fusarium graminearum is found worldwide as a pathogen of cereal grains, in particular of wheat and maize, and it produces a mycotoxin known as deoxynivalenol (DON or vomitoxin). Each year, the presence of this compound and related trichothecenes causes substantial losses to agricultural productivity. Rapid methods for the measurement of the toxin in grains are required to monitor and divert effectively contaminated grain from the food supply. A fluorescence polarization (FP) immunoassay using a previously described monoclonal antibody for DON was developed. The assay was based on the competition of unlabeled DON from a sample with a fluorescently tagged DON, DON-fluorescein (DON-FL), for a DON-specific monoclonal antibody in solution. The FP of the tagged DON was increased upon binding with the antibody. In the presence of free toxin, less of the DON-FL was bound and the polarization signal was decreased. The assays were very simple to perform, requiring only mixing of an aqueous extract of wheat with the DON-FL and antibody. The sensitivity of the assay was strongly dependent upon the time between mixing of the sample with the tracer and measurement of the fluorescence polarization, with midpoints for the competition curves ranging from 0.03 µgml-1 with a 15-s incubation to >1 µgml-1 with a 12-min incubation. Samples of wheat naturally contaminated with DON were evaluated by FP and by an HPLC-UV method, with a good correlation (r 2 = 0.97). Although the FP method tended to overestimate DON slightly in the wheat samples, by ˜20%, the assay was easy to use and very useful for the screening of wheat.
Full Text
Topics from this Paper
Determination Of Deoxynivalenol In Wheat
Fluorescence Polarization
Monoclonal Antibody In Solution
Deoxynivalenol In Wheat
Related Trichothecenes
+ Show 5 more
Create a personalized feed of these topics
Get StartedSimilar Papers
Journal of Agricultural and Food Chemistry
Feb 26, 2002
Food Analytical Methods
Apr 28, 2013
Journal of Agricultural and Food Chemistry
Jan 6, 2001
Mycopathologia
Apr 1, 2006
Analytical and Bioanalytical Chemistry
Sep 8, 2011
Journal of AOAC INTERNATIONAL
Nov 1, 1983
Journal of AOAC INTERNATIONAL
Sep 1, 1986
World Mycotoxin Journal
Aug 1, 2008
Journal of AOAC INTERNATIONAL
Jul 1, 1984
Journal of AOAC INTERNATIONAL
Jan 1, 1984
Fresenius' Journal of Analytical Chemistry
Apr 25, 2000
Journal of AOAC INTERNATIONAL
Jan 1, 1986
Journal of Agricultural and Food Chemistry
Jul 22, 2010
Biosensors
Oct 25, 2020
Toxins
Nov 6, 2014
Food Additives & Contaminants
Food Additives & Contaminants
Jan 1, 2008
Food Additives & Contaminants
Dec 1, 2007
Food Additives & Contaminants
Dec 1, 2007
Food Additives & Contaminants
Dec 1, 2007
Food Additives & Contaminants
Dec 1, 2007
Food Additives & Contaminants
Dec 1, 2007
Food Additives & Contaminants
Dec 1, 2007
Food Additives & Contaminants
Dec 1, 2007