Abstract
Optical properties of a fluorescence molecule can be drastically changed by surface plasmons excited in neighboring metallic nanostructures. Here we investigated the fluorescence enhancement behavior of fluorescein isothiocyanate (FITC) labeled polystyrene nanoparticles coupled to silver island films (SIFs) via a 15 nm polymethyl methacrylate separation layer theoretically and experimentally. Up to 24-fold fluorescence enhancement was experimentally achieved when the annealing time of the 25 nm Ag films was 50 min, which is in good agreement with the theoretical simulation result based on the finite-difference time-domain method. Furthermore, significant fluorescence spectral distortion on SIFs was also observed compared with samples on glass slides, which is sufficiently related to the scattering properties of SIFs and the lifetimes of FITC.
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