Abstract
The presence of formaldehyde-induced fluorescence in malignant melanoma cells, junctional naevus cells and normal melanocytes has been confirmed in freeze-dried material. It has been found that the same fluorescence can be observed in formalin-fixed “routine” paraffin sections. Microspectrofluorimetry showed that the characteristics of the fluorescence are similar in all 3 types of cells, with excitation and emission maxima at about 440 and 490 nm respectively. The fluorescence excitation maximum is not affected by hydrochloric acid. It is suggested that fluorescence microscopy of formalin fixed sections is a simple and valuable diagnostic method.
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