Fluorescence microscope by using computational ghost imaging

Yasuhiro Mizutani,Kyuki Shibuya,Tetsuo Iwata,Yasuhiro Takaya,Y Bellouard

doi:10.1051/matecconf/20153205001

Yasuhiro Mizutani, Kyuki Shibuya + Show 3 more

Open Access

https://doi.org/10.1051/matecconf/20153205001

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Abstract

We propose a fluorescence microscope by using the computational Ghost imaging (CGI) for observ- ing a living cell for a long duration over an hour. There is a problem for observing a cell about light-induced bleaching for a ling-term observation. To over come the problem, we focused on an advantage of sensitivity of the CGI as second order colleration for an imaging with weak intensity excitation light. Setting for the CGI, a DMD projector was installed at an eye-piece part of a microscope and fluorescent light was detected using a bucket detector of a photo-multiplier tube. As a results, we have shown the imaging advantage of the CGI under weak light intensity, in addition, we have demonstrated to detect fluorescence images of biological samples for one day.

Highlights

Fluorescence-microscopic imaging is a powerful tool for analyzing in the biological field for the past decades[1]
We have converted a commercial microscope to a computational Ghost imaging (CGI) microscope by attaching a digital mirror device (DMD) projector and a bucket detector
The DMD projector(Texas Instruments Inc./ DLP Lightcrafter) and a photomultiplier tube(Hamamatsu Photonics K.K./ H6780-01) as the bucket detector were installed at a eyepiece part of the microscope(Nikon Corp./ ECLIPSE TE200U) and the side of a sample stage, respectively

Summary

Introduction

Fluorescence-microscopic imaging is a powerful tool for analyzing in the biological field for the past decades[1]. The spacial resolution is one of the most important objects for development of the fluorescence microscope, the sensitivity is important problem for observing biological samples because there is light-induced bleaching. By using the GI or CGI as fluorescence-imaging methods, there is an advantage for weak intensity imaging because a sensitivity of a bucket detector is higher than 2D detectors and there is a multiplex effect[9]. We propose a possibility to observe for weak intensity of the fluorescence by using the CGI, theoretically and experimentally. For using the fluorescence CGI (FCGI), we first give the advantage of detecting the weak intensity in the view point of the contrast-to-noise ratio and present the fluorescence imaging of biological samples for long-term observation without photobleaching

Computational Ghost imaging for fluorescent microscope

Quantitative analysis for advancement of detecting weak light

Experimental results

Conclusions