Abstract
An interface based on Multichannel scaler (MCS; SR430; Stanford Research Systems, USA) has been tested for enabling fluorescence lifetime measurements of pO2-sensitive fluorophores with a custom-made confocal scanning microfluorometer (CSMF) for transcorneal pO2 measurements. SR430 served as a transient photon counter. A trigger pulse, coincident with the rising edge of an excitation pulse for fluorescence, was coupled to accumulate emission pulses detected by a photomultiplier tube (PMT; R928 HA, Hamamatsu, Inc). The pulse started the photon counting and segmented the photon count data into consecutive time bins (ranging from 1 up to 32k), the width of which could be varied from 5 ns to 10.486 ms. With no dead time between bins, photon counting with the fluorescence decay is seamless. Silica-bound Ruthenium phenanthroline, dispersed in silicone rubber, was employed as a fluorophore sensitive to pO2. The test interface for CSMF-MCS system showed sensitive pO2 measurements and hence is suitable to measure local pO2 at any depth across the cornea.
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