Abstract
Ribonucleic acids (RNAs) play a central biological role across all domains of life. RNA localization, biomolecular association, and dynamics of these processes are linked and closely influence RNA function. For quantitative insight into these processes, it is essential to visualize RNAs live and track responses to cellular perturbations. In this study, we established FLIM for live RNA tracking. Our genetically encoded RNA FLIM sensor builds upon the Riboglow platform. Riboglow consists of a small molecule fluorescent probe that binds a genetically encoded RNA tag and changes fluorescence via quenching upon binding.
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