Fluorescence Imaging Topography Scanning System for intraoperative multimodal imaging.

Tri T Quang,Francis A Papay,W Barry Edwards,Forrest Sheng Bao,Hye-Yeong Kim,Irene Georgakoudi,Yang Liu

doi:10.1371/journal.pone.0174928

Tri T Quang, Francis A Papay + Show 5 more

Open Access

https://doi.org/10.1371/journal.pone.0174928

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Abstract

Fluorescence imaging is a powerful technique with diverse applications in intraoperative settings. Visualization of three dimensional (3D) structures and depth assessment of lesions, however, are oftentimes limited in planar fluorescence imaging systems. In this study, a novel Fluorescence Imaging Topography Scanning (FITS) system has been developed, which offers color reflectance imaging, fluorescence imaging and surface topography scanning capabilities. The system is compact and portable, and thus suitable for deployment in the operating room without disturbing the surgical flow. For system performance, parameters including near infrared fluorescence detection limit, contrast transfer functions and topography depth resolution were characterized. The developed system was tested in chicken tissues ex vivo with simulated tumors for intraoperative imaging. We subsequently conducted in vivo multimodal imaging of sentinel lymph nodes in mice using FITS and PET/CT. The PET/CT/optical multimodal images were co-registered and conveniently presented to users to guide surgeries. Our results show that the developed system can facilitate multimodal intraoperative imaging.

Highlights

Multimodal imaging is a promising strategy to overcome limitations of medical imaging technologies by combining strengths of individual modalities
We have developed a novel Fluorescence Imaging Topography Scanning (FITS) system
We have demonstrated the concept of FITS system, the instrumentation design and the feasibility of using the system for intraoperative multimodal imaging and surgical navigation

Summary

Introduction

Multimodal imaging is a promising strategy to overcome limitations of medical imaging technologies by combining strengths of individual modalities. Multimodal imaging modalities such as positron emission tomography and x-ray computed tomography (PET/CT) and single photon emission computed tomography and x-ray computed tomography (SPECT/ CT) are used to capture and present both functional and anatomical data to physicians, facilitating clinical decision making. Their utility in intraoperative settings is limited due to their high cost and the extended scanning time [1,2,3,4,5,6].

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