Fluorescence image and microbiological analysis of biofilm retained around healthy and inflamed orthodontic miniscrews.

Abstract

Peri-miniscrew inflammation is one of the causes of orthodontic miniscrew failure. The aim of this study was to correlate and quantify throughout autofluorescence images, PCR and microbiologic count of biofilm retained around orthodontic miniscrew and the presence of Porphyromonas gingivalis. Forty miniscrews used for orthodontic treatment were evaluated during orthodontic treatment, collected from patients and divided into two groups: healthy and inflamed miniscrews. To be considered inflamed, the samples should present: loss of stability checked by periotest®, clinical presence of mucositis, red aspect of the gum or bleeding around the miniscrew. Immediately after removal of the miniscrews, they were photographed using a macro 100 lens and a Pentax camera coupled to a fluorescent equipment - Qscan (AioBio - Korea) with 405 nm excitation wavelength and a blue band filter. A microbiologic sample was collected with a sterile microbrush scrubbed around the miniscrew tread. Fluorescent images were analyzed with ImageJ software to quantify fluorescent intensity and fluorescent area and microbiological samples were submitted to CFU count for total contamination and q-PCR assay to quantify Porphyromonas gingivalis. The results showed a good correlation between CFU count and fluorescent intensity and PCR/fluorescent area. The healthy miniscrews presented less fluorescent intensity and lower CFU count when compared to inflamed miniscrews. q-PCR analysis showed a higher number of P. gingivalis contamination around inflamed miniscrews. Quantification of biofilm retained by miniscrew by images of autofluorescence is a simple and reliable method with great potential for clinical use to monitory inflammation around miniscrew and risk of loss.