Abstract
Spraying of the fluorescence spot on a thin-layer plate with a non-volatile and viscous organic solvent after development enhanced the fluorescence. The elucidation of the physicochemical factors that affect such an enhancing effect was investigated. The effect was not related to the transmissibility of light through the thin layer but to the properties of the spraying reagent (polarity, viscosity and acidity). The appropriate reagents for 5-dimethylamino-1-naphthalenesulphonamides (DANS-amines) are non-polar, viscous and non-acidic solvents such as a mixture of liquid paraffin and n-hexane, which enhanced the fluorescence internsity 10-fold. With benzo( a)pyrene, the use of a solvent such as this liquid paraffin mixture enhanced the fluorescence intensity 35-fold.
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