Abstract
Fluorescence energy transfer in lipid vesicles between N-(7-nitrobenz-2-oxa-1,3,-diazol-4-yl-labelled phosphatidylethanolamine (acting as donor) and N-(lissamine-rhodamin B)-labelled phosphotidylethanolamine (acting as acceptor) was studied by steady state and time-resolved fluorescence quenching analysis. Both fluorescent phospholipids were incorporated as minor components in four different types of lipid vesicle: dipalmitoylphosphatidylglycerol vesicles in their L β gel phase at 20 °C and in their L α liquid crystalline phase at 50 °C, and egg yolk phosphatidylethanolamine vesicles at 40 °C in their L α liquid crystalline phase at pH 9.5 and in their H II inverted hexagonal phase at pH 5.0. The quenching of the donor fluorescence by energy transfer is diffusion controlled in all cases, except in the L β gel phase. The dimensionality and type of constraints imposed on diffusion are different in each case, with the most efficient diffusion-controlled quenching in the hexagonal phase.
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