Abstract
Abstract A liquid chromatographic procedure for the simultaneous determination of mexiletine and two major metabolites, p-hydroxymexiletine and hydroxymethylmexiletine, in plasma and urine is described. A reversed-phase ion-pair C16column is used with gradient elution using post-column fluorescence derivatization with o-phthalaldehyde. The lower limits of detection are 2 ng/ml for mexiletine and p-hydroxymexiletine and 5 ng/ml for hydroxymethylmexiletine. Enzymatic hydrolysis of conjugate forms of oxidative metabolites in urine samples was also performed. The coefficients of variation for replicate assay of spiked samples were uniformly less than 10% for all the analytes.
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