Abstract
Modern high-performance liquid chromatography (HPLC) can provide high sensitivity and selectivity for the measurement of peptides which have been suitably derivatized for fluorescence detection. Several approaches to the chemical modification of peptides for high sensitivity fluo-rescence detection in HPLC have been developed and are routinely useful for low level determinations. Under optimal conditions, HPLC with fluorescence detection can provide sensitivity for peptides comparable with that of immunoassay techniques, and, in some cases, affords additional selectivity. An example of the utility of HPLC approaches for the high sensitivity determination of disulfide- and arginine-containing peptides in biological samples in support of pharmacokinetic studies is described.
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