Abstract
The use of fluorescence emission to distinguish between pathologic tissue of tumor and blood vessel was demonstrated. Spectroscopic guidance angioplasty has been reported. These are all based on the difference between normal and pathologic tissue fluorescence. Laser-induced biological fluorescence (LIF) depends on excited wavelength specimen environment. In this work a fourth harmonic from Nd:YAG laser is used as a excitation source. Fluorescence spectra were observed from normal wall of human and animal. Laser-induced fluorescence were also reported from cancerous tissues and cells. We have analyzed the dependence of the fluorescence on specimen environment and exciting intensity. Reasons of causing the difference of LIF were discussed.
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