Abstract
An enzyme-coupled fluorometric assay for uric acid has been developed, applicable over a wide range of uric acid concentrations and a pH range of 6 to 8, and capable of measuring picomole quantities. The assay is dased upon the preference of horseradish peroxidase for uric acid rather than scopoletin as a substrate. The method has been applied to serum, urine, and liver tissue. Some practical advantages and wider applications of this method are discussed as well as some possible physiological ramifications of the enzyme reaction upon which this assay is based.
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