Abstract
Fluorescence anisotropy imaging and sensing is a widely recognized method for studying molecular orientation and mobility. However, introducing this technique to in vivo systems is a challenging task, especially when one considers multiphoton excitation methods. Past two decades have brought a possible solution to this issue in the form of hollow-core antiresonant fibers (HC-ARFs). The continuous development of their fabrication technology has resulted in the appearance of more and more sophisticated structures. One of the most promising concepts concerns dual hollow-core antiresonant fibers (DHC-ARFs), which can be used to split and combine optical signals, effectively working as optical fiber couplers. In this paper, the design of a fluorescence anisotropy sensor based on a DHC-ARF structure is presented. The main purpose of the proposed DHC-ARF is multiphoton-excited fluorescence spectroscopy; however, other applications are also possible.
Highlights
Hollow core antiresonant fibers (HCARFs) attract a great deal of researchers’ attention due to their remarkable optical features such as several transmission bands over which they exhibit low losses, and low nonlinearities and low dispersion [1]
Where λk is the wavelength of the considered transmission window, d0 is the thickness of the first layer of the high-index material surrounding the core, ng is the material’s refractive index and k is the number of the transmission window (0,1,2, . . . )
HCARF-based sensors employing non-linear methods for medical diagnostics up to this day lack one interesting functionality—namely the capability of measuring fluorescence anisotropy
Summary
Hollow core antiresonant fibers (HCARFs) attract a great deal of researchers’ attention due to their remarkable optical features such as several transmission bands over which they exhibit low losses, and low nonlinearities and low dispersion [1]. The second, known as dual-channel configuration, employs two photodetectors, one for each of the polarizations, allowing for their simultaneous observation These anisotropy detection schemes are often integrated with fluorescence microscopy systems. Only a few solutions of FA measurement setups with an in-vivo potential have been presented [35,36,37] These methods require special calibration schemes in order to compensate for depolarization factors such as telescope optics. The of hollow-core splitting signals with different polarizations using has manner would beidea a dual antiresonant fiber (DHC-ARF). Its parameters have been analyzed, allowing to determine its possible use in a multiphoton excited polarization-splitting parameters have been analyzed, allowing to determine its possible use in a fluorescence anisotropy setup.
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