Abstract

Rutin is one of the most promising flavonoid from a pharmacological and biochemical point of view. Here we have explored its spectroscopic and photophysical properties at room temperature and 77K using steady-state absorption-luminescence methods and pulse spectroscopy equipment. By excitation into the absorption band 1 of rutin in methanol at room temperature the normal Stokes׳ shifted fluorescence with a maximum at 415nm and quantum yield of 2×10−4 was revealed. However, by excitation into the bands 2 and 3 any emission wasn’t observed. At 77K in ethanol glass we have observed fluorescence at 410nm and phosphorescence at 540nm for the first time. As a result the adequate energetic scheme including the lowest electronic excited singlet at 26000cm−1 and triplet at 19600cm−1 states was proposed.

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