Fluorescence and Bioluminescence Genetically Encoded Probes for Imaging Protein Conformational Changes and Interactions in Living Cells and Animals

Abstract

Rapid advancement in fluorescence and luminescence imaging technology is encouraging cell biologists to develop high-throughput assays for intracellular detection of bioactive small molecules, protein conformational changes, protein localization and dynamics, and measurement of protein—protein interactions in real time at the level of single living cells with high spatial and temporal resolution. In this report, we summarize the development of genetically encoded fluorescent and bioluminescent probes to study various molecular events in live cells and animals for potential drug candidates against different diseases.