Abstract
A method is described for the isolation of homogeneous populations of principal and intercalated cells (PC and ICC) of the rabbit cortical collecting duct using fluorescence-activated cell sorting (FACS) in tandem with solid phase immunoadsorption. Three sorting strategies are described. In the first one the two cell types are separated based on the different intensities of their reaction with a monoclonal antibody. In the second and third strategies PC and ICC are tagged with cell-specific markers coupled to different fluorochromes and separated based on their green and red or green and blue fluorescence, respectively. These near homogeneous (∼99%) populations of PC and ICC can be obtained in numbers large enough to perform biochemical characterization, determination of hormonal sensitivity and initiation of cell cultures.
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