Flunarizine enhances functional recovery following sciatic nerve crush lesion in rats

Abstract

We have used the rat sciatic nerve crush (SNC) injury model to assess the neuroprotective effects of flunarizine (FNZ), a calcium channel antagonist and a vasodilator. The animals were treated with FNZ for various durations following SNC (0.33 mg/kg per day, i.p). Employing the physical disector method, we quantitated the rates of neuron loss in the dorsal root ganglion and spinal cord and protective effects of FNZ. FNZ treatment following SNC reduced neuron loss up to 86.6 and 82.5% in DRG sensory and spinal cord motor neurons, respectively. Functional recovery following SNC with or without FNZ treatment was assessed using the measurements of the total, 1–5 and 2–4-toe spread to quantitate percentage relative toe spread in relation to the respective controls. FNZ provided a superior return of function, i.e. near absolute recovery of both sensory and motor functions in 4 weeks, which is consistent with its neuroprotective effects.