Abstract
Fluid-phase endocytosis in rainbow trout macrophages was studied using 125 I-poly (vinylpyrrolidone) (125I-PVP) as a marker. Uptake of 125I-PVP was measurable at 12° C using a high cell concentration (30-50 × 106 cell ml-1) and after extensive washing of the cells by centrifugation. The amount of extracellular fluid taken up after 30 min incubation with labelled PVP was estimated as 3.6±2.2 nl/106 cells, corresponding to a fluid uptake of approximately 1·4% of the cell volume per hour. Uptake of 125 I-PVP was temperature dependent and was not saturable after coincubation with excess unlabelled PVP. Fluid-phase endocytosis of labelled PVP did not follow a linear time course. This seemed to be caused by exocytosis of internalised 125I-PVP to the medium.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
