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Abstract
Fluid-phase endocytosis in rainbow trout macrophages was studied using 125 I-poly (vinylpyrrolidone) (125I-PVP) as a marker. Uptake of 125I-PVP was measurable at 12° C using a high cell concentration (30-50 × 106 cell ml-1) and after extensive washing of the cells by centrifugation. The amount of extracellular fluid taken up after 30 min incubation with labelled PVP was estimated as 3.6±2.2 nl/106 cells, corresponding to a fluid uptake of approximately 1·4% of the cell volume per hour. Uptake of 125 I-PVP was temperature dependent and was not saturable after coincubation with excess unlabelled PVP. Fluid-phase endocytosis of labelled PVP did not follow a linear time course. This seemed to be caused by exocytosis of internalised 125I-PVP to the medium.
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