Fluid dynamic parameters of naturally derived hydroxyapatite scaffolds for in vitro studies of bone cells

Abstract

Hydroxyapatite scaffolds obtained from the biomorphic transformation of wood are characterized from a fluid dynamic point of view. Such material of recent introduction offers great advantages for the in vitro study of bone cells, mostly in virtue of its peculiar porous structure. Determining the flow resistance and morphological parameters of these scaffolds is an essential step towards their practical use in bioreactors and microfluidic devices. To this aim, a series of tests involving a draining fluid are performed on a set of disc-shaped scaffolds, followed by the microscopy analysis of the pores visible on the sample faces. Contrarily to what expected, a temperature dependence is observed for the flow resistance, even after normalizing it by the fluid properties. The interpretation of the experimental results is assisted by numerical outcomes from Computational Fluid Dynamics modelling, which underline some limitations in the application of classical laws to the present problem. While the complex and variable internal structure of the scaffolds prevents the systematic use of simplified formulae, a correlation is found between the flow resistance and the pore geometry, which can facilitate the characterization of further samples.