Flufenoxuron, an acylurea insect growth regulator, alters development of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) by modulating levels of chitin, soluble protein content, and HSP70 and p34 cdc2 in the larval tissues

Abstract

The effect of sublethal concentrations, 0.00141% (LC 20), 0.00251% (LC 30), and 0.0036% (LC 40) of a dispersible formulation of an acylurea insect growth regulator, flufenoxuron (Cascade) on certain biochemical parameters in the larvae of Tribolium castaneum was investigated. When neonates were fed on diet treated with sublethal concentrations for 24 h, it was observed that at all concentrations tested, there was a significant reduction in chitin content on the 15th day of development. Total soluble protein content at LC 20 and LC 30 decreased with increasing age of the larvae. At LC 20 and LC 40 concentrations there was a progressive increase in the protein: chitin ratio as a function of increase in age of the larvae. SDS–PAGE analysis of the larval tissue extracts indicated gross quantitative changes in some of the protein bands (MW 50–97 kDa). Western blot analysis revealed significant increase in the level of HSP70 in the extracts of larvae fed on LC 30 treated diet, on the 7th and 10th day of development in the decreasing order. Conversely, a significant decrease in the hyper-phosphorylated form of p34 cdc2 kinase due to flufenoxuron treatment indicating modulation of cell cycle regulation was observed. Thus, sublethal concentrations of flufenoxuron alter expression of developmentally regulated proteins, HSP70 and p34 cdc2 and chitin formation in a stage-specific manner thereby resulting developmental abnormalities in T. castaneum.