Abstract
Formamide hydrolase activity in a barley field soil was described by a two-component, rather than a one-component model of Michaelis–Menten kinetics. The two-component model had both a high-affinity ( K m values of 0.5–1.0 mM) and a low-affinity ( K m values of 30–60 mM) activity. Rapid and transient increases in both the overall heterotrophic activity (CO 2 production) and the high-affinity component of formamide hydrolase activity were observed in rewetted soil samples, suggesting that the high-affinity component represented enzyme activity of active microorganisms. Short-term field variations in the high-affinity component during a barley growth season were consistent with rapid, transient responses of microbial activity to precipitation and soil wetting. An assay of the high-affinity component of formamide hydrolase activity as approximated by rate determination at a single low (1 mM) formamide concentration can provide an easy and rapid indication of enzyme activity related directly to active microorganisms in the soil.
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