Fluctuations during growth of the plasma membrane H(+)-ATPase activity of Saccharomyces cerevisiae and Schizosaccharomyces pombe.

Abstract

The plasma membrane H(+)-ATPase activity was determined under various growth conditions using the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. Under early batch-growth conditions in a rich medium, the budding yeast S. cerevisiae ATPase specific activity increased 2- to 3-fold during exponential growth. During late exponential growth, a peak of ATPase activity, followed by a sudden decrease, was observed and termed "growth-arrest control". The growth arrest phenomenon of S. cerevisiae could not be related to the acidification of the culture medium or to glucose exhaustion in the medium or to variation of glucose activation of the H(+)-ATPase. Addition of ammonium to a proline minimum medium also stimulated transiently the ATPase activity of S. cerevisiae. Specific activity of the fission yeast S. pombe ATPase did not show a similar profile and steadily increased to reach a plateau in stationary growth. Under synchronous mitotic growth conditions, the ATPase activity of S. cerevisiae increased during the cell division cycle according to the "peak" type cycle, while that of S. pombe was of the "step" type.