Abstract
Properties of aluminium fluoride (A1F) complex-activated nonselective cation (NS) channels in guinea-pig chromaffin cells were investigated using the patch clamp technique. As the membrane potential was hyperpolarized from the holding potential of −55 mV, the A1F-induced nonselective cation current (INS) diminished progressively. With hyperpolarizations to −100 MV or more negative potentials, the A1F INS almost instantaneously disappeared. The apparent unit conductance of A1F INS was estimated to be 3 pS by fluctuation analysis. The open state probability of A1F-activated NS channels became large with a decrease in concentration of free Mg2+ ions inside the cell and was less than 0.5 at 12 μM Mg2+. It is concluded that NS channels in the chromaffin cell apparently differ from those in smooth muscle cells.
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