Abstract
We present a novel single-cell analyzer, flow-lysometry, to measure cytosolic components in large cell populations. The present flow-lysometry performs and synchronizes three functions (cell-detection, cell-lysis, and component-sensing) in a continuous microfluidic channel, thus achieving high-throughput measurements (20 cells/min) of wide target components. In the experimental study, we measure the cytosolic component (Ca++ ion) from each single RBC and verify population analysis of single cells in mixed cell populations. Thus, this work shows that the present flow-lysometry is useful to characterize complex cell populations, which is required for various biomedical studies.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
